Absolutely. Here's what you do: First, connect your computer or laptop to the EZbus via USB. Next, connect the MIDI out of your controller keyboard to either of the MIDI inputs on the EZbus. Now, when you play a note on your controller, that MIDI signal goes out of EZbus via USB and triggers the virtual synth. Here's where it gets fun: The audio from the virtual synth comes back into a couple of EZbus channels via USB, and that signal is mixed with your regular hardware synths (which are plugged into other EZbus channels). Your entire keyboard rig---real and virtual---is now a single, integrated system!