First, try un/reinstalling the MOTU. Remove it from the Add/Remove Programs in Control Panel, do not restart computer when it asks, and go to Device Manager and right-click, Uninstall the 3 MOTU drivers.

Then reinstall the drivers.

If that does not work, make sure the PCI324 Asio.dll file is in your C:\Windows\System32 directory. It should be approximately 89 KB.