Yes and No. If the Analog I/O cards are put into the inner 2 slots (where the Digital I/O card and Expansion slot normally are) the cards will not be recognized unless they are reconnected to their original ribbon cables. However, because of heat issues the (Analog I/O cards being hotter than the Digital I/O card) it is likely that operating a 192 like this will shorten the life of the Analog cards.